I am using magnetic beads on dropbot chip. For making a proper mixing/mixture of bio-reagent buffers with Magnetic Beads, I have tried different ways/pattern of mixing on dropbot chip (like clock-wise mixing, anti-clock wise mixing, both clock-wise and anti clockwise mixing, horizontal and vertical way of mixing by selecting some electrodes in ‘+’ shape) with a delay of 1500ms, 700ms. All the mixing experiments had a minimum of 10 rounds of mixing. But I haven’t get a proper ‘mixture’. The Magnetic Beads were placed in one ‘corner’ inside the droplet after mixing.
The specifications of Magnetic Beads which we are using includes :
‘The grain size of the magnetic particle is about 70 nm. Magnetic particle aggregated by some granule and its average diameter is about 100-500 nm.’
Is there any better way for making a Mixture of Buffer while using Magnetic Beads ?
Thanks in advance.